Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources

Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources

Plant secondary metabolites have been attracting people's attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using micr...

Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway

BACKGROUND Vanillin is an industrially valuable molecule that can be produced from simple carbon sources in engineered microorganisms such as Saccharomyces cerevisiae and Escherichia coli. In E. coli, de novo production of vanillin was demonstrated previously as a proof of concept. In this study, a series of data-driven experiments were performed in order to better understand limitations associ...

Simulation of Bioreactors for PHB Production from Natural Gas

Recently, many economic studies of poly(3-hydroxybutyrate) PHB production on an industrial scale, and the impact of replacing petrochemical polymers by PHB were carried out, clearly indicating that the most crucial factors to reduce the cost of producing biopolymers are allotted to the application of microbial production strains capable of high productivi...

De novo sphingolipid biosynthesis: a necessary, but dangerous, pathway.

Heurstic search for metabolic engineering: de novo synthesis of vanillin

We present the application of heuristic search to in silico metabolic pathway engineering. In particular, we discuss a new computational approach to elucidate complex pathways and to address the practical challenge of combinatorial complexity in pathway inference. We have implemented this approach in a new computational framework, called PathMiner, which is useful for designing metabolic engine...